According to the experience of the old tenant, "If you want to burst out, you can prepare the ten winter wax, and the first month can only sweep the tail." Therefore, it is the time to prepare for planting. It is a bacterium of sclerotia and is used for medicinal purposes. It is mainly used in Anhui, Fujian, Hubei, Hunan and other provinces. It is warm, dry, ventilated, sunny and abundant. The slope is required to be around 10-30 degrees, and the soil is sandy loam, fertile and humid environment. Wild crickets usually grow at a height of more than 500 meters and grow on decaying pine roots or pines about 20 cm underground.
The growth and development of alfalfa can be divided into two stages, the mycelial growth stage and the sclerotial formation stage. The mycelial growth stage is mainly that the hyphae absorb water and nutrients from the pine, and multiply a large number of vegetative hyphae. When the mycelium accumulates more, it becomes a sclerotium, and when it is formed into a sclerotium stage, it is called a knotting stage. Artificial cultivation of cockroaches is currently commonly used in the cultivation method of sorghum cultivation. First, the pure sputum strain should be cultivated, and then the sorghum should be selected.
Cultivation of pure strains
(1) Preparation of culture medium for the seedlings: Potato-agar medium is used, and the formula is 250 g of potato, 50 g of sucrose, 20 g of agar 8, 3 g of urea and 1000 ml of water; Adjust the pH to 6-7, pack in a test tube, dress it, sterilize it, and make it into a slant medium. Isolation and inoculation of pure strains: Select fresh, thin, fleshy, tight, scented mature sclerotia as a strain, first wash with water, then move into the inoculation box; 75% of the month rinse Rinse with distilled water or cold water several times, blot dry with filter paper, cut with a knife, pick up a small piece of central white meat, place it on the inclined surface of the medium, and put it into a 25-30 degree incubator or culture. The medium is cultured for 5-7 days, and when the white hyphae are covered with a slant, it is a pure species.
(2) Cultivation of original species
After successful breeding of the purebred seed, it cannot be directly used for production, and it is necessary to expand the breeding and breeding of the original species. The original medium formula: 55% of small pine block, 20% of pine wood chips, 20% of rice bran or wheat bran, 4% of sucrose, 1% of gypsum powder; add appropriate amount of water to adjust pH to 5-6. Preparation method: first mix the wood chips, rice bran, gypsum powder; further dissolve the sucrose with water, put it in the pine block and boil for 30 minutes, after the pine block fully absorbs the sugar liquid, remove it; then the shavings, rice sugar; gypsum powder Pour into the liquid, mix well; then add the wooden block, mix well, to the fingers of the grip can not sew water. It is then dispensed into a 500 ml jar, sterilized, and then cooled. Inoculation method: Under aseptic conditions, the mother fungus species of soybean size is selected from the parent species and placed in the center of the jar original seed, which is the original species.
(3) Cultivation of cultivars
The cultivar culture medium is composed of 66% wood block, 10% pine wood chips, 21% wheat bran or fine glutinous rice, 2% glucose, 1% gypsum powder, 0.4% urea, 1% superphosphate, water added, pH adjusted. 5-6. The preparation method is the same as above. The hyphae in the original seed are connected to a jar containing the cultivation material, and the hyphae are overgrown for about 30 days. 1 bottle of pure seed can be 5-8 bottles of original species, 1 bottle of original species can be 60 bottles of cultivars, 1 bottle of cultivar can be connected to 2-3 inches.
Selection and preparation of pine wood
Choose sandy loam with deep and loose soil and good drainage, sunny slopes and uncultivated wasteland and woodland without any crops. The hoe will be dug in winter, with a length of 80 cm, a width of 45 cm and a depth of 30 cm. The spacing is 30 cm and the drainage ditch is opened.
Using pine as the cultivation material, cut down the pine trees in the lunar calendar in December, go to the tree stalks, cut off the outer skin of the layer, expose the xylem, peel off the ribs, saw the wooden segments 60-80 cm long, dry after stacking, about After 40 days, no rosin is secreted, and the heart can be inoculated after drying.
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